Clone | Ustekinumab | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Catalog # | SIM0020 | ||||||||||||
Category | InVivoSIM™ Biosimilar Antibodies | ||||||||||||
Price |
|
This non-therapeutic biosimilar antibody uses the same variable regions from the therapeutic antibody Ustekinumab making it ideal for research use. This Ustekinumab biosimilar reacts with human p40 also known as IL-12β. p40 is a 40 kDa subunit of IL-12 and IL-23. IL-12 is a heterodimeric cytokine composed of subunits IL-12α p35 and IL-12β p40. The p40 subunit of IL-12 also combines with p19, a protein that shows no biological activity by itself, to form IL-23. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells while IL-23 is secreted by activated dendritic cells and epithelial cells. IL-12 plays roles in T lymphocyte differentiation, IFNγ production, and NK cell cytotoxicity. The C17.8 antibody has been shown to neutralize both IL-12 and IL-23 bioactivity. Ustekinumab is used for the treatment of Crohn’s disease, plaque psoriasis, and psoriatic arthritis.
Isotype | Human IgG1, κ |
---|---|
Recommended Isotype Control(s) | InVivoPlus human IgG1 isotype control |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Immunogen | Human IL-12 p40 |
Reported Applications | Functional assays |
Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
Aggregation | <5% Determined by DLS |
Purity | >95% Determined by SDS-PAGE |
Sterility | 0.2 µm filtration |
Production | Purified from tissue culture supernatant in an animal free facility |
Purification | Protein A |
Molecular Weight | 150 kDa |
Murine Pathogen Tests | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |