InVivoMAb anti-mouse/human phosphorylated PD-1 (CD279)

Clone 407.6G12
Catalog # BE0387
Category InVivoMab Antibodies
Price
Size Regular Price
1mg $ 159.00
5mg $ 583.00
25mg $ 1,950.00
50mg $ 2,936.00
100mg $ 4,155.00
About InVivoMAb anti-mouse/human phosphorylated PD-1 (CD279)

The 407.6G12 monoclonal antibody reacts with phosphorylated mouse PD-1. Specifically, the antibody only reacts with PD-1 when tyrosine 248 (Y248) is phosphorylated. PD-1 is a 50-55 kDa cell surface receptor that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. PD-1 signals via binding its two ligands, PD-L1 and PD-L2. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Phosphorylation of Y248 of the ITSM motif of PD-1 is essential for the initiation of inhibitory signals. PD-1 blockade leads to reduced phosphor-PD-1. The 6G12 antibody can be used to assess the efficacy of PD-1 pathway blockade. 

InVivoMAb anti-mouse/human phosphorylated PD-1 (CD279) Specifications
IsotypeMouse IgG2a, κ
Recommended Isotype Control(s)InVivoMAb mouse IgG2a isotype control, unknown specificity
Recommended Dilution BufferInVivoPure pH 7.0 Dilution Buffer
ImmunogenHuman PD-1 p248Tyr peptide conjugated to KLH
Reported ApplicationsWestern Blot
Flow Cytometry
FormulationPBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin<2EU/mg (<0.002EU/μg)
Determined by LAL gel clotting assay
Purity>95%
Determined by SDS-PAGE
Sterility0.2 µm filtration
ProductionPurified from tissue culture supernatant in an animal free facility
PurificationProtein G
Molecular Weight150 kDa
StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Application References